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Volume 13, Issue 6 , Pages 717-721 , November 2009

Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China

Jianguo Chen
- Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Zhaoliang Su
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Yingzhao Liu
- Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
Shengjun Wang
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Xiaoli Dai
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Yazhen Li
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Sufang Peng
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Qixiang Shao
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Haifang Zhang
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Ping Wen
- Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
Jianren Yu
- Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
Xinxiang Huang
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
Huaxi Xu
- Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
- Corresponding author. Tel.: +86 511 85038140; fax: +86 511 85038449.

Received 6 July 2008 ,Revised 22 September 2008 ,Accepted 26 November 2008.

Schematic representation of the 1.6-kb integron digested with HinfI and EcoRV. M, DNA marker; 1, 1.6-kb integron PCR amplicon; 2–4, ZJ070, ZJ199, and ZJ374 digested with HinfI; 5–7, ZJ070, ZJ199, and

Schematic representation of the 1.6-kb integron digested with HinfI and EcoRV. M, DNA marker; 1, 1.6-kb integron PCR amplicon; 2–4, ZJ070, ZJ199, and ZJ374 digested with HinfI; 5–7, ZJ070, ZJ199, and ZJ374 digested with EcoRV.
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Schematic representation of the class 1 integrons from P. aeruginosa isolates. These integrons had approximate sizes of (A) 2.5kb, (B) 1.6kb, and (C) 0.75kb. Arrows indicate the direction of transcrip

Schematic representation of the class 1 integrons from P. aeruginosa isolates. These integrons had approximate sizes of (A) 2.5kb, (B) 1.6kb, and (C) 0.75kb. Arrows indicate the direction of transcription. The core is represented by black circles.
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PFGE of SpeI-digested chromosomal DNA. M, molecular size marker; lanes 1–22, P. aeruginosa isolates from different patients. PFGE patterns A, B, C, and D of the isolates are shown.

PFGE of SpeI-digested chromosomal DNA. M, molecular size marker; lanes 1–22, P. aeruginosa isolates from different patients. PFGE patterns A, B, C, and D of the isolates are shown.
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