International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages 717-721 , November 2009

Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China

  • Jianguo Chen

      Affiliations

    • Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Zhaoliang Su

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Yingzhao Liu

      Affiliations

    • Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
    • ,
  • Shengjun Wang

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Xiaoli Dai

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Yazhen Li

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Sufang Peng

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Qixiang Shao

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Haifang Zhang

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Ping Wen

      Affiliations

    • Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
    • ,
  • Jianren Yu

      Affiliations

    • Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China
    • ,
  • Xinxiang Huang

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • ,
  • Huaxi Xu

      Affiliations

    • Department of Microbiology and Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang, Jiangsu Province, 212013 China
    • Corresponding Author InformationCorresponding author. Tel.: +86 511 85038140; fax: +86 511 85038449.

Received 6 July 2008 ,Revised 22 September 2008 ,Accepted 26 November 2008.

References 

  1. Sarlangue J, Brissaud O, Labrèze C. Clinical features of Pseudomonas aeruginosa infections. Arch Pediatr. 2006;13(Suppl 1):S13–S16
  2. Aarestrup FM, Wegener HC. The effects of antibiotic usage in food animals on the development of antimicrobial resistance of importance for humans in Campylobacter and Escherichia coli. Microbes Infect. 1999;1:639–644
  3. Rowe-Magnus DA, Mazel D. Resistance gene capture. Curr Opin Microbiol. 1999;2:483–488
  4. Weldhagen GF. Integrons and β-lactamases—a novel perspective on resistance. Int J Antimicrob Agents. 2004;23:556–562
  5. Thungapathra M, Amita , Sinha KK, Chaudhuri SR, Garg P, Ramamurthy T, et al. Occurrence of antibiotic resistance gene cassettes aac(6′)-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. Antimicrob Agents Chemother. 2002;46:2948–2955
  6. Héritier C, Dubouix A, Poirel L, Marty N, Nordmann P. A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58. J Antimicrob Chemother. 2005;55:115–118
  7. Leverstein-van Hall MA, Blok HE, Donders AR, Paauw A, Fluit AC, Verhoef J. Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. J Infect Dis. 2003;187:251–259
  8. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 16th Information supplement; M100-S16. Wayne, PA: CLSI; 2006.
  9. Hall RM, Collis CM. Antibiotic resistance in Gram-negative bacteria: the role of gene cassettes and integrons. Drug Resist Updates. 1998;1:109–119
  10. Shahada F, Chuma T, Tobata T, Okamoto K, Sueyoshi M, Takase K. Molecular epidemiology of antimicrobial resistance among Salmonella enterica serovar Infantis from poultry in Kagoshima, Japan. Int J Antimicrob Agents. 2006;28:302–307
  11. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995;33:2233–2239
  12. Tenover FC, Filpula D, Phillips KL, Plorde JJ. Cloning and sequencing of a gene encoding an aminoglycoside 6′-N-acetyltransferase from an R factor of Citrobacter diversus. J Bacteriol. 1988;170:471–473
  13. Casin I, Bordon F, Bertin P, Coutrot A, Podglajen I, Brasseur R, et al. Aminoglycoside 6′-N-acetyltransferase variants of the Ib type with altered substrate profile in clinical isolates of Enterobacter cloacae and Citrobacter freundii. Antimicrob Agents Chemother. 1998;42:209–215
  14. Livermore DM. beta-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995;8:557–584
  15. White PA, McIver CJ, Deng Y, Rawlinson WD. Characterization of two new gene cassettes, aadA5 and dfrA17. FEMS Microbiol Lett. 2000;182:265–269
  16. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Infect Control. 2006;34:S3–S10
  17. Gu B, Tong M, Zhao W, Liu G, Ning M, Pan S, et al. Prevalence and characterization of class I integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing, China. J Clin Microbiol. 2007;45:241–243
  18. Fonseca EL, Vieira VV, Cipriano R, Vicente AC. Class 1 integrons in Pseudomonas aeruginosa isolates from clinical settings in Amazon region, Brazil. FEMS Immunol Med Microbiol. 2005;44:303–309
  19. Zhang H, Shi L, Li L, Guo S, Zhang X, Yamasaki S. Identification and characterization of class 1 integron resistance gene cassettes among Salmonella strains isolated from healthy humans in China. Microbiol Immunol. 2004;48:639–645
  20. Chang LL, Chang TM, Chang CY. Variable gene cassette patterns of class 1 integron-associated drug-resistant Escherichia coli in Taiwan. Kaohsiung J Med Sci. 2007;23:273–280
  21. Kang HY, Jeong YS, Oh JY, Tae SH, Choi CH, Moon DC, et al. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. J Antimicrob Chemother. 2005;55:639–644
  22. Lindstedt BA, Heir E, Nygard I, Kapperud G. Characterization of class I integrons in clinical strains of Salmonella enterica subsp. enterica serovars Typhimurium and Enteritidis from Norwegian hospitals. J Med Microbiol. 2003;52:141–149
  23. Nógrády N, Gadó I, Tóth A, Pászti J. Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003. Int J Antimicrob Agents. 2005;26:126–132
  24. Guerra B, Junker E, Schroeter A, Malorny B, Lehmann S, Helmuth R. Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. J Antimicrob Chemother. 2003;52:489–492

PII: S1201-9712(09)00003-4

doi: 10.1016/j.ijid.2008.11.014

International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages 717-721 , November 2009

Article Tools

* Image Usage & Resolution