International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages e463-e466, November 2009

Bilateral periprosthetic joint infection caused by Salmonella enterica serotype Enteritidis, and identification of Salmonella sp using molecular techniques☆

  • Hideo Kobayashi

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
    • ,
  • Gerri S. Hall

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • ,
  • Marion J. Tuohy

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • ,
  • Ulf Knothe

      Affiliations

    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
    • ,
  • Gary W. Procop

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • ,
  • Thomas W. Bauer

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 216 444 6830; fax: +1 216 445 6967.

Received 29 September 2008; received in revised form 17 November 2008; accepted 15 December 2008. published online 09 March 2009.

Corresponding Editor: William Cameron, Ottawa, Canada

Summary 

Salmonella septic arthritis is rare. Our objective was to identify bacterial species from joint fluid using a broad-range real-time PCR and pyrosequencing technique. We describe a case of bilateral Salmonella enterica serotype Enteritidis infection of right and left total knee arthroplasties. DNA was extracted from the joint fluid of the left knee, amplified by PCR, and the amplicons were evaluated by pyrosequencing. The patient was treated with ciprofloxacin, and the polyethylene liners were replaced in both knees. The results of pyrosequencing detected a Salmonella species. To the best of our knowledge, this is the first report describing the detection of Salmonella in joint fluid by universal PCR followed by pyrosequencing.

Keywords: Salmonella, Joint fluid, Total knee arthroplasty, Real-time polymerase chain reaction, Pyrosequencing

 

☆ This manuscript was presented in part as: “Joint infection caused by Salmonella enteritidis, and identification of Salmonella using broad-range PCR and pyrosequencing technology”, at the 108th General Meeting of the American Society for Microbiology, Boston, June 1–5, 2008.

PII: S1201-9712(09)00066-6

doi:10.1016/j.ijid.2008.12.015

International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages e463-e466, November 2009

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