International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages e463-e466 , November 2009

Bilateral periprosthetic joint infection caused by Salmonella enterica serotype Enteritidis, and identification of Salmonella sp using molecular techniques

  • Hideo Kobayashi

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
  • ,
  • Gerri S. Hall

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
  • ,
  • Marion J. Tuohy

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
  • ,
  • Ulf Knothe

      Affiliations

    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
  • ,
  • Gary W. Procop

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
  • ,
  • Thomas W. Bauer

      Affiliations

    • Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA
    • Orthopaedic and Rheumatologic Institute, The Cleveland Clinic, Cleveland Ohio, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 216 444 6830; fax: +1 216 445 6967.

Received 29 September 2008 ,Revised 17 November 2008 ,Accepted 15 December 2008.

  • Image Result

    A Quantification mode of real-time PCR. Two Salmonella enterica serotype Enteritidis samples in our laboratory (*) were amplified at around 10 cycles. The amplification of the clinical sample DNA occu

    A Quantification mode of real-time PCR. Two Salmonella enterica serotype Enteritidis samples in our laboratory (*) were amplified at around 10 cycles. The amplification of the clinical sample DNA occurred at 16 cycles. (B) The pyrogram of the case. This pyrogram is read as follows, “CTCACAGTTC……”. This sequence was categorized as Salmonella enterica according to BLAST.

 This manuscript was presented in part as: “Joint infection caused by Salmonella enteritidis, and identification of Salmonella using broad-range PCR and pyrosequencing technology”, at the 108th General Meeting of the American Society for Microbiology, Boston, June 1–5, 2008.

PII: S1201-9712(09)00066-6

doi: 10.1016/j.ijid.2008.12.015

International Journal of Infectious Diseases
Volume 13, Issue 6 , Pages e463-e466 , November 2009